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Bala Krishna Manikyam

G. Venkateswara Rao

Abstract

Currently, the pursuit of plant extracts exhibiting potent antimicrobial properties has heightened. As a result of Tinospora sinensis being employed in traditional medicine for combating inflammatory and infectious conditions, this research assessed the in vitro antimicrobial capabilities and phytochemical makeup of extracts derived from its stem and leaves. Phytochemical screening was conducted utilizing established reference methods. The antimicrobial activities of various extracts were assessed through the agar well diffusion method, while the antibiotic susceptibility of selected microorganisms was examined using the disc diffusion method. The preliminary examination of the phytochemical profile reveals that the various extracts in the formulation encompass a wide array of vital phytochemicals, including alkaloids, flavonoids, glycosides, cardiac glycosides, tannins, phenols, steroids, quinones, proteins, carbohydrates, and saponins. The leaf and stem displayed sufficient activity, with the leaf exhibiting the highest level of activity. The chloroform extracts showed the highest activity, followed by aqueous, acetone, ethyl acetate, and methanol extracts. Staphylococcus aureus was found to be more susceptible to both leaf and stem extracts, followed by Bacillus subtilis, Pseudomonas fluorescens, and Streptococcus mutans, respectively, at a concentration of 10mg/l. These findings support the exploration of active components that may serve as lead molecules in the creation of novel antimicrobial medications.

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